Glycogen phosphorylase inhibition improves beta cell function
نویسندگان
چکیده
منابع مشابه
Liver Glycogen Phosphorylase Kinase
Liver glycogen phosphorylase kinase was assayed by measuring the radioactivity of [y-32P]ATP incorporated into rabbit skeletal muscle phosphorylase b. Using this assay two forms of phosphorylase kinase, Kinase I and Kinase II, were pirified about 70and 130-fold, respectively, from rabbit liver cytosol by DEAE-Sephadex A50 column chromatography, ammonium sulfate fractionation, gel filtration on ...
متن کاملGlycogen Phosphorylase in DictyosteLium discoideum
The glycogen phosphorylase of Dictyostelium discoideum has been purified over 200-fold from cells in the culmination stage of development. Analytical gel electrophoresis of the purified enzyme indicates one major protein band with a molecular weight of approximately 210,000. Gel elution verified the presence of phosphorylase activity associated with the protein band. Electrophoresis of partiall...
متن کاملComparative Studies on Glycogen Phosphorylase
The isolation and crystallization of both forms of glycogen phosphorylase from human skeletal muscle (1) have made possible a detailed study of the properties of these enzymes in comparison with those of rabbit skeletal muscle (2, 3). Phosphorylase b and a from both species have been shown to crystallize under similar conditions and to have similar pH optima, electrophoretic mobilities, sedimen...
متن کاملORDERED MECHANISM OF BINDING OF PHOSPHORYLASE KINASE AND GLYCOGEN PHOSPHORYLASE b TO GLYCOGEN
The kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen has been studied using turbidimetric method (40 mM Hepes, pH 6.8, and 8.2; 20◦C). The binding of phosphorylase kinase with glycogen occurs only in the presence of Ca and Mg . According to the kinetic data, phosphorylase b favors the binding of phosphorylase kinase with glycogen. This conclusion is suppo...
متن کاملPhosphodiesterase 5 Inhibition Improves β-Cell Function in Metabolic Syndrome
OBJECTIVE This study tested the hypothesis that phosphodiesterase 5 inhibition alone or in combination with ACE inhibition improves glucose homeostasis and fibrinolysis in individuals with metabolic syndrome. RESEARCH DESIGN AND METHODS Insulin sensitivity, beta-cell function, and fibrinolytic parameters were measured in 18 adults with metabolic syndrome on 4 separate days after a randomized,...
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ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 2017
ISSN: 0007-1188
DOI: 10.1111/bph.13819